Summary of Microarray Experiments

Experiments performed on rat U34A Affymetrix chips:

Time course experiment for cytokine-induced genes in INS-1E cells:
INS-1E cells were exposed for six different time points: 1, 2, 4, 8, 12
or 24 hours to IL-1β (10 U/ml) + IFNγ(100 U/ml) +/- the inducible NO
synthase (iNOS) blocker N(G)-monomethyl-L-arginine (NMA) NMA (1.0
mmol/l). Results are means of 2 experiments for each time point. 698
cytokine-responsive genes will be included by February 2005 in T1DBase
from this experiment.

• Kutlu B, Cardozo AK, Darville MI, Kruhoffer M, Magnusson N, ORntoft T, Eizirik DL. Discovery of Gene Networks Regulating Cytokine-Induced Dysfunction and Apoptosis in Insulin-Producing INS-1 Cells. (2003) Diabetes. 52:2701-19 PMID: 14578289
  

Cytokine-induced genes in primary rat beta-cells:
FACS-purified rat beta cells were exposed for 6 hours or 24 hours to
IL-1beta (50U/ml) or a combination of IL-1beta (50U/ml) and IFN-gamma
(1000U/ml). Results are fold change compared to controls and represent
means of 2 chip determinations of one pool from 4-6 independent
experiments or in the case of IL-1beta and IFN-gamma, mean
determination from 2 pools from 4-6 independent experiments, each
analyzed in duplicate.

• Cardozo AK, Kruhøffer M, Leeman R, Orntoft T, Eizirik DL. Identification of novel cytokine-induced genes in pancreatic beta-cells by high-density oligonucleotide arrays. (2001) Diabetes. 50:909-20 PMID: 11334433
  
• Cardozo AK, Heimberg H, Heremans Y, Leeman R, Kutlu B, Kruhøffer M, Ørntoft T, Eizirik DL. A comprehensive analysis of cytokine-induced and nuclear factor-kappa B-dependent genes in primary rat pancreatic beta-cells. (2001) J Biol Chem. 276:48879-86 PMID: 11687580
  

Double stranded RNA- and IFN-gamma-induced genes in primary rat beta cells:
FACS-purified rat beta cells were exposed for 6 hours or 24 hours to
IFN-γ (1000U/ml) and/or Poly-I:C (polyinosinic-polycytidylic acid; 100
µg/ml). Results are fold change compared to controls and represent
means of 2 chip determinations of one pool from 4-6 independent
experiments.

• Rasschaert J, Liu D, Kutlu B, Cardozo AK, Kruhøffer M, ØRntoft TF, Eizirik DL. Global profiling of double stranded RNA- and IFN-gamma-induced genes in rat pancreatic beta cells. (2003) Diabetologia. 46:1641-57 PMID: 14600816
  

Experiments performed on human U133 Affymetrix chips:

Global profiling of coxsackievirus- and cytokine-induced gene expression in human pancreatic islets:
Isolated human islets were exposed for 48h to either IL-1β (50 U/ml)
and IFN-γ (1000 U/ml) or CBV-5 (coxsackievirus 5B; multiplicity of
infection 30-100). Results are fold change compared to controls and
represent means of 3 chip determinations from 3 individual donor
samples.

• Ylipaasto P, Kutlu B, Rasilainen S, Rasschaert J, Salmela K, Teerijoki H, Korsgren O, Lahesmaa R, Hovi T, Eizirik DL, Otonkoski T, Roivainen M. Global profiling of coxsackievirus- and cytokine-induced gene expression in human pancreatic islets. (2005) Diabetologia. 48:1510-22 PMID: 15991020